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De Cegli Rossella

Short Biography

Education
  • 1997-2002 “Laurea cum laude” Degree in Biological Science at University of Naples “Federico II” (5 year University studies).
  • 2002 Qualification of Biologist from the Italian Association of Biologists.
  • 2002-2006 Ph.D. Degree from the School of “Biochemistry and Molecular and Cellular Biology” at University of Naples “Federico II” (4 year graduate Ph.D. program).
  • 2005-2006 Visiting Graduate Student in Prof.Yang Shi laboratory at Harvard Medical School, Boston (MA).
  • 2006-2012 PostDoc at TIGEM (Italy) in the laboratory of Prof. Andrea Ballabio.
  • 2012-today Grant Researcher (Assegnista di Ricerca; four years-long).
Technical Skills
  • Excellent theoretical and practical knowledge of molecular biology methods and techniques of recombinant DNA and RNA technology.
  • Excellent theoretical and practical knowledge of the basic techniques of biochemistry and extensive experience working on proteins.
  • Extensive experience and manual skills with different types of cell cultures coming from human or other organisms.
  • Extensive experience and manuality with mouse embryonic stem cells.
  • Good knowledge of different differentiation protocols into the three germ layers expecially in the differentiation in Neurons and Glia cells.
  • Isolation of primary hepatocytes from mice.
  • Excellent theoretical and practical knowledge of different bioinformatics tools for the analysis of gene expression, gene characterization and gene networks.
  • Excellent theoretical and practical knowledge of bioinformatics tools for the analysis of protein structures and domains.
Publications
  • Medugno L., Florio F., De Cegli R., Grosso M., Lupo A., Costanzo P. and Izzo P. “The Kruppel-like zinc-finger protein ZNF224 represses aldolase A gene transcription by interacting with the KAP-1co-repressor protein”. Gene, 359 35– 43 (2005) (27 citations).
  • Lan F., Collins R, De Cegli R, Alpatov R., Horton J.R., Xiaobing Shi X., Gozani O., Cheng X. and Shi Y. “ Recognition of unmethylated histone H3 lysine 4 links BHC80 to LSD1-mediated gene repression”. Nature, 448 (2007) (279 citations).
  • Cesaro E., De Cegli R., Medugno L., Florio F., Grosso M., Lupo A., Izzo P. and Costanzo P. “The Kruppel-like zinc finger protein ZNF224 recuits the arginine mathyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene”. J Biol Chem, 284(47): 32321-30 (2009). Epub 2009 Sep 9 (26 citations).
  • De Cegli R., Romito A., Iacobacci S., Mao L., Lauria M., Fedele A.O., Klose J., Borel C., Descombes P., Antonarakis S.E., di Bernardo D., Banfi S., Ballabio A. and Cobellis G. “A mouse Embryonic Stem Cell Bank for inducible overexpression of human Chromosome 21 genes”. Genome Biology, 11:R64 (2010) (11 citations).
  • Lei Mao, Rossella De Cegli, Mario Lauria, Grit Nebrich, Jean Maurice Delabar, Yann Herault, Gilda Cobellis and Joachim Klose. Chapter 20 “Tracking the Structure of Protein Interaction Network via Multiple Genetic Perturbations on Mouse Embryonic Stem Cells — Implementation of the Entropy Maximization Principle” in the book “Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Applications”, ISBN 978-953-307-197-8, edited by Craig Atwood (1 citation).
  • De Cegli R, Iacobacci S, Flore G, Gambardella G, Mao L, Cutillo L, Lauria M, Klose J, Illingworth E, Banfi S, di Bernardo D. Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation. Nucleic Acids Res. 2012 Nov 23 (10 citations).
  • Settembre C, De Cegli R, Mansueto G, Saha PK, Vetrini F, Visvikis O, Huynh T, Carissimo A, Palmer D, Jurgen Klisch T, Wollenberg AC, di Bernardo D, Chan L, Irazoqui JE, Ballabio A. TFEB controls cellular lipid metabolism through a starvation-induced autoregulatory loop. Nature Cell Biology 2013 Apr 21 (151 citations).
  • Gambardella G, Moretti MN, De Cegli R, Cardone L, Peron A, di Bernardo D.Differential Network Analysis for the identification of condition-specific pathway activity and regulation. Bioinformatics 2013 Jun 6 (21 citations).
  • Giuseppina Di Fruscio, Angela Schulz, Rossella De Cegli, Marco Savarese, Margherita Mutarelli, Giancarlo Parenti, Sandro Banfi, Thomas Braulke, Vincenzo Nigro, and Andrea Ballabio. Lysoplex: An efficient toolkit to detect DNA sequence variations in the autophagy-lysosomal pathway. Autophagy. 2015;11(6):928-38. (8 citations).
  • G. Di Fruscio , M. Savarese, R. De Cegli, M. Mutarelli, V. Singhmarwah, D. Di Bernardo, S. Banfi, A. Ballabio, V. Nigro . LysoPlex: a “preferential exome” strategy to clarify molecular mechanisms of autophagic vacuolar myopathies Abstracts / Neuromuscular Disorders 24 (2014) 791–924
  • Alessia Calcagnì, Lotte Kors, Eric Verschuren, Rossella De Cegli, Nicolina Zampelli, Edoardo Nusco, Stefano Confalonieri, Giovanni Bertalot, Salvatore Pece, Carmine Settembre, Gabriel G. Malouf1, Jaklien C. Leemans, Emile de Heer, Marco Salvatore, Dorien J.M. Peters, Pier Paolo Di Fiore and Andrea Ballabio. Modelling TFE renal cell carcinoma in mice reveals a critical role of WNT signaling. Elife. 2016 Sep 26;5.
  • Mansueto G, Armani A, Viscomi C, D'Orsi L, De Cegli R, Polishchuk EV, Lamperti C, Di Meo I, Romanello V, Marchet S, Saha PK, Zong H, Blaauw B, Solagna F, Tezze C, Grumati P, Bonaldo P, Pessin JE, Zeviani M, Sandri M, Ballabio A. Transcription Factor EB Controls Metabolic Flexibility during Exercise. Cell Metab. 2017 Jan 10;25(1):182-196. doi: 10.1016/j.cmet.2016.11.003. Epub 2016 Dec 20.
  • Chiara Di Malta, Diletta Siciliano, Alessia Calcagni, Jlenia Monfregola, Simona Punzi, Nunzia Pastore, Andrea N. Eastes, Oliver Davis, Rossella De Cegli, Angela Zampelli, Luca G. Di Giovannantonio, Edoardo Nusco1, Nick Platt, Alessandro Guida, Margret Helga Ogmundsdottir, Luisa Lanfrancone, Rushika M. Perera, Roberto Zoncu, Pier Giuseppe Pelicci, Carmine Settembre, Andrea Ballabio. Transcriptional activation of RagD GTPase controls mTORC1 and promotes cancer growth. Science 16 Jun 2017.